What is Incubation of Cells with Annexin V-FITC and PI?
As a new method for detecting apoptosis, the Annexin V binding assay is based on the measurement of the loss of plasma membrane asymmetry. Under normal physiological conditions, cell sustain a strictly asymmetric distribution of phospholipids in the two leaflets of the cellular membranes, where phosphatidylserine (PS) facing the cytosolic side.