Premade AAV Particles
The Adeno-Associated Virus (AAV) is a non-enveloped, small single-stranded DNA Parvovirus that commonly infects humans but has no known pathology. Its genome contains two open reading frames, Rep and Cap, which are flanked by two inverted terminal repeats (ITR). In addition to Rep and Cap, AAV requires a helper plasmid containing the adenovirus genes E2a, E4, and VA. These genes mediate the AAV replication.